Anti-PI 3 Kinase p85 alpha antibody (HRP Conjugated)
- 100 μL
产品信息
蛋白质全称 | 磷脂酰肌醇3-激酶调节亚基α |
别名 | PIK3R1, AGM7, GRB1, IMD36, P85, p85-ALPHA, Phosphoinositide-3-kinase regulatory subunit 1, PI 3 Kinase p85 alpha, pi3k, PI3K |
Uniprot ID | P27986 |
免疫原 | 人PI 3 Kinase p85 alpha重组蛋白 |
预测分子量./观测分子量. | 85 kDa / 85 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人 | 1: 500-1: 1000 | U-87 MG细胞, SK-OV-3细胞 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗PI3 Kinase p85 alpha 抗体(ZB11525-HRP)在室温(20-25℃)孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
图像
| WB检测PI 3 Kinase p85 alpha蛋白(货号 ZB11525-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA(货号GC305010)的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗PI 3 Kinase p85 alpha蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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