产品信息

蛋白质全称	丝裂原活化蛋白激酶3
别名	APK1, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, MAPK1,MAPK3, P42MAPK, P44MAPK, PRKM1, PRKM2, p44, p42, p44-MAPK, p42-MAPK, P-ERK, P-ERK1/2, P-ERK1, P-ERK2
Uniprot ID	P27361
免疫原	KLH偶联的人ERK1 (T202/Y204) + ERK2 (T185/Y187)合成磷酸肽
抗体亚型	IgG
预测分子量./观测分子量.	41/43 kDa / 42/44 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 2000	PC12细胞, HT29细胞, NIH 3T3细胞

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背景

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

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图像

	WB检测Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187)蛋白(货号 GB113492). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187)蛋白(货号 GB113492). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.