产品信息

蛋白质全称	果糖二磷酸醛缩酶C
别名	Aldolase 3, Brain-type aldolase, Scrapie-responsive protein 2, Zebrin II, Aldoc, Aldo3, Scrg2, ALDOC, ALDC, Aldolase C, Aldolase, Fructose-bisphosphate C
Uniprot ID	P05063
免疫原	KLH偶联的小鼠Aldolase C合成肽
抗体亚型	IgG
预测分子量./观测分子量.	40 kDa / 40 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	脑, 大脑皮层, 海马区

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背景

ALDOC is one of the three aldolase isozymes (A, B, and C), encoded by three different genes. Aldolase C (fructose bisphosphate aldolase) is a key enzyme in the fourth step of glycolysis, as well as in the reverse pathway gluconeogenesis. It catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehydes-3-phosphate (G3P), or glyceraldehyde, and dihydroxyacetone phosphate (DHAP) by aldol cleavage. As a result, it is a crucial player in ATP biosynthesis.

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图像

WB检测Aldolase C蛋白(货号 GB11825). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.