产品信息

蛋白质全称	胞外丝氨酸/苏氨酸蛋白激酶FAM20C
别名	Dentin matrix protein 4, DMP 4, DMP4, FAM20C, Protein FAM20C, RNS
Uniprot ID	Q5MJS3
免疫原	KLH偶联的小鼠FAM20C合成肽
抗体亚型	IgG
预测分子量./观测分子量.	66 kDa / 70 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	大鼠	1: 800-1: 1500	卵巢, 肝

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背景

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth . Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome (By similarity). Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity (By similarity). Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1 . In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (By similarity).

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图像

WB检测FAM20C蛋白(货号 GB115127). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.