产品信息

蛋白质全称	cGMP特异性3',5'-环磷酸二酯酶
别名	Pde5a, Pde5, cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE, CN5A, phosphodiesterase 5A, phosphodiesterase isozyme 5, PDE5A1
Uniprot ID	Q8CG03
免疫原	小鼠PDE5A重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	98 kDa / 105 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	肝, 肺, 肌肉

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背景

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

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图像

WB检测PDE5A/PDE5蛋白(货号 GB111522). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.