产品信息

蛋白质全称	干扰素诱导双链RNA激活蛋白激酶
别名	eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase R, Pkr, Eif2ak2, Prkr, p68 kinase, Tik, Serine/threonine-protein kinase TIK, Tyrosine-protein kinase EIF2AK2
Uniprot ID	Q03963
免疫原	小鼠PKR重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	58 kDa / 62 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	脾, 脑, 心

---

背景

PKR is an IFN-induced serine/threonine protein kinase consisting activated by dsRNA produced during viral infections, PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth and differentiation. Upon activation, PKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway.

---

图像

WB检测PKR蛋白(货号 GB111097). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.