产品信息

蛋白质全称	Delta样蛋白4
别名	Delta like protein 4, delta like 4 (Drosophila), Delta4, DLL4, Drosophila Delta homolog 4, hdelta2, AOS6, delta like canonical
Uniprot ID	Q9JI71
免疫原	KLH偶联的小鼠DLL4合成肽
抗体亚型	IgG
预测分子量./观测分子量.	74 kDa / 75 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 300-1: 400	脑

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背景

Involved in the Notch signaling pathway as Notch ligand . Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting . Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types ). During spinal cord neurogenesis, inhibits V2a interneuron fate.

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图像

WB检测DLL4蛋白(货号 GB115572). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.