Anti-Src Rabbit pAb
- 50 μL
产品信息
蛋白质全称 | 原癌基因Src酪氨酸蛋白激酶 |
别名 | Proto-oncogene c-Src, pp60c-src, p60-Src, Src, ASV, Avian sarcoma virus, Tyrosine protein kinase SRC1, SRC1, SRC proto-oncogene, non receptor tyrosine kinase, THC6 |
免疫原 | 小鼠Src重组蛋白 |
Uniprot ID | P05480 |
抗体亚型 | IgG |
纯化方式 | 亲和纯化 |
预测分子量. / 观测分子量. | 60 kDa / 55 kDa |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠, 大鼠 | 1: 500-1: 1000 | 脑, 睾丸 |
背景
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase super family, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
图像
| WB检测Src蛋白(货号 GB112343). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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