产品信息

蛋白质全称	II型细胞角蛋白1
别名	67 kDa cytokeratin, Cytokeratin-1, Keratin-1, CK-1, K1, Type-II keratin Kb1, Krt1, Krt2-1, EHK1, Keratin type II cytoskeletal 1
Uniprot ID	P04264
免疫原	KLH偶联的人Cytokeratin 1合成肽
抗体亚型	IgG1, κ
克隆号	SB144
预测分子量./观测分子量.	66 kDa / 66 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	A-431细胞

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背景

Cytokeratin 1 is a heterotetramer of two type I as well as two type II keratins localized in the cell membrane and is generally associated with keratin-10 . It has been suggested to regulate the activity of kinases such as PKC/SRC via binding to integrin beta-1 and the receptor of activated protein kinase C. Cytokeratin 1 and its heterodimer partner KRT10 are the major constituents of 4 intermediate filament cytoskeleton in suprabasal epidermis/keratinocytes, and their upregulation precedes expression of filaggrin and cornified envelope proteins.

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图像

WB检测Cytokeratin 1蛋白(货号 GB151017). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.