产品信息

蛋白质全称	P2Y嘌呤受体12
别名	ADP glucose receptor, ADPG-R, Purinergic receptor P2RY12, Platelet ADP receptor, HORK3, BDPLT8, P2Y12, P2ry12
Uniprot ID	Q9H244
免疫原	人P2RY12重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	39 kDa / 50 kDa
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HMC3细胞, SH-SY5Y细胞
IHC/IF 免疫组织化学/免疫荧光	人	1: 200-1: 800	胶质瘤, 脑, 胶质母细胞瘤

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背景

The P2Y receptor family are G protein-coupled receptors which mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C. To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. Human platelets express two G protein-coupled nucleotide receptors, P2Y1 and P2Y12.

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图像

	WB检测P2Y12蛋白(货号 GB115742). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	IHC检测P2Y12蛋白(货号 GB115742). 样品: 人胶质母细胞瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测P2Y12蛋白(货号 GB115742). 样品: 人神经胶质瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测P2Y12蛋白(货号 GB115742). 样品: 人胶质母细胞瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测P2Y12蛋白(货号 GB115742). 样品: 人神经胶质瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测P2Y12蛋白(货号 GB115742). 样品: 人神经胶质瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测P2Y12蛋白(货号 GB115742). 样品: 人神经胶质瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IF analysis of P2RY12(GB115742) red. sample: Human brain, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human brain, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human glioblastoma, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human glioblastoma, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human glioblastoma, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human glioma tumor, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human glioma tumor, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of P2RY12(GB115742) red. sample: Human glioma tumor, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: P2RY12(GB115742) red 1: 200, 4℃ overnight. Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. Iba 1 (GB12102) green 1: 500, 4℃ overnight. 488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.