产品信息

蛋白质全称	细胞周期蛋白A2
别名	Cyclin-A, Ccna2, CycA, CycA2, Cyclin A2,Cyclin A, CCNA2, CCNA, CCN1
Uniprot ID	P51943
免疫原	KLH偶联的小鼠Cyclin A2合成肽
抗体亚型	IgG1, κ
克隆号	SB160
预测分子量./观测分子量.	49 kDa / 55 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HT-29细胞, A549细胞, K562细胞

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背景

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.

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图像

WB检测Cyclin A2蛋白(货号 GB151228). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗:1: 1000, 4℃ 孵育过夜. 二抗:HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.