产品信息

蛋白质全称	神经元迁移蛋白双皮质素
别名	DBCN, Dbct, DC, DCX, Doublecortex, Doublin, Lis-X, Lissencephalin X, SCLH, XLIS, LISX, doublecortin
Uniprot ID	O88809
免疫原	KLH偶联的小鼠Doublecortin合成肽
抗体亚型	IgG1, κ
克隆号	SB72
预测分子量./观测分子量.	41 kDa / 41 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1000-1: 2000	脑（新生）, 胎脑

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背景

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

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图像

WB检测Doublecortin蛋白(货号 GB15317). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.