产品信息

蛋白质全称	神经细胞粘附分子1
别名	N-CAM-1, NCAM-1, CD56, Ncam1, Ncam, MSK39, antigen MSK39 identified by monoclonal antibody 5.1H11, NCAMC
Uniprot ID	P13591
免疫原	人重组NCAM1蛋白（293F细胞表达）
抗体亚型	IgG2b, κ
克隆号	2G8C6
纯化方式	亲和纯化
亚细胞定位	细胞膜, 分泌型

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人	1: 600-1: 1300	结肠

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背景

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.

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图像

IHC检测NCAM1蛋白(货号 GB12041). 样品: 人结肠, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 650稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.