产品信息

蛋白质全称	丝裂原活化蛋白激酶8/9/10
别名	JNK1 + JNK2 + JNK3,JNK1/2/3,JNK1+2+3,MAPK8, MAPK9,MAPK10,c Jun N terminal kinase 1,c Jun N terminal kinase 2,c Jun N terminal kinase 3
Uniprot ID	P45983, P45984, P53779
免疫原	人JNK1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	46 ,54 kDa / 46 ,54 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 2000	脑, 心

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背景

JNK1(MAPK8) is a member of the MAP kinase family. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53. JNK3 is a neuron-specific form of c-Jun N-terminal kinases. Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis.

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图像

WB检测JNK1+JNK2+JNK3蛋白(货号 GB114321). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.