Recombinant Anti-Phospho-PI3K p85 (Y467)+PI3K p55 (Y199) antibody (Rabbit mAb)
- 50 μL
产品信息
蛋白质全称 | 磷脂酰肌醇 3-激酶调节亚基α |
别名 | PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, p85, pi3k p85, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha (PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha),p55,pi3k p55,P55G,PIK3R3,Phosphatidylinositol 3-kinase regulatory subunit gamma,PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma |
Uniprot ID | P27986 |
免疫原 | KLH偶联的人PI3R1合成磷酸肽 |
抗体亚型 | IgG |
克隆号 | SB684 |
预测分子量./观测分子量. | 84 kDa / 55/85 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | 经渗透钠酸盐处理的NIH/3T3 |
背景
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
图像
| WB检测Phospho-PI3K p85 (Y467)+PI3K p55 (Y199)蛋白(货号 GB150210). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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