产品信息

蛋白质全称	抗原肽转运体2
别名	ATP-binding cassette sub-family B member 3, Histocompatibility antigen modifier 2, APT2, Tap2, Abcb3, Ham-2, Ham2, ABC18, PSF2, RING 11, Peptide transporter TAP2
Uniprot ID	P36371
免疫原	KLH偶联的小鼠TAP2合成肽
抗体亚型	IgG
预测分子量./观测分子量.	77 kDa / 77 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 600	HeLa细胞, MCF-7细胞, U-2 OS细胞

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背景

TAP is an integral transmembrane protein involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It is a heterodimer of TAP1 and TAP2, and the peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. TAP is inducible by interferon gamma and belongs to the ABC transporter family, MDR subfamily. TAP also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide.

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图像

WB检测TAP2蛋白(货号 GB113273). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.