Anti-SIX3 Rabbit pAb
- 100 μL
产品信息
蛋白质全称 | 同源框蛋白SIX3 |
别名 | HPE2, Sine oculis homeobox homolog 3, SIX homeobox 3 |
Uniprot ID | Q62233 |
免疫原 | KLH偶联的小鼠Six3合成肽 |
抗体亚型 | IgG |
预测分子量./观测分子量. | 36 kDa / 37 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠 | 1: 100-1: 300 | C2C12细胞, SH-SY5Y细胞, 293T细胞 |
背景
Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon . Acts as a direct upstream activator of SHH expression in the rostral diencephalon ventral midline and that in turn SHH maintains its expression . In addition, Six3 activity is required for the formation of the telencephalon.Acts on the proliferation and differentiation of neural progenitor cells through activating transcription of CCND1 AND CCND2 .
图像
| WB检测SIX3蛋白(货号 GB114815). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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