产品信息

蛋白质全称	CREB调节转录共激活因子1
别名	Mucoepidermoid carcinoma translocated protein 1 homolog, TORC-1, Transducer of CREB protein 1, Crtc1, Kiaa0616, Mect1, Torc1, CRTC1, MECT1, TORC1, WAMTP1, CREB regulated transcription coactivator 1, MAML2, Mam-2
Uniprot ID	Q68ED7
免疫原	KLH偶联的小鼠TORC1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	67 kDa / 78 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 小脑, 海马区

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背景

CRTC1, also named as MECT1, TORC1, and WAMTP1, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. CRTC1 acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. It enhances the interaction of CREB1 with TAF4. CRTC1 regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. It is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells.

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图像

WB检测TORC1蛋白(货号 GB111000). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.