Anti-HMGB1 antibody (HRP Conjugated)
- 100 μL
产品信息
蛋白质全称 | 高迁移率族蛋白B1 |
别名 | HMGB1, HMG1, HMG3, SBP-1, HMG-1, high mobility group box 1 |
Uniprot ID | P63158 |
免疫原 | KLH偶联的小鼠HMGB1合成肽 |
预测分子量./观测分子量. | 25 kDa / 25 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | NIH3T3细胞, 肾, PC-12细胞 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗HMGB1 抗体(ZB11103-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts withnucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription.After binding, HMGB1 bendsDNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes.
图像
| WB检测HMGB1蛋白(货号 ZB11103-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗HMGB1蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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