产品信息

蛋白质全称	乙酰辅酶 A 合成酶（胞质型）
别名	AceCS, ACSS2, ACAS2, Acyl-activating enzyme, Acetate--CoA ligase, Acetyl-CoA synthetase, ACS, Acyl-CoA synthetase short-chain family member 2, ACSA, Acetate--CoA ligase, Acetyl-CoA synthetase
Uniprot ID	Q9QXG4
免疫原	KLH偶联的小鼠ACSS2合成肽
抗体亚型	IgG
预测分子量./观测分子量.	79 kDa / 79 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	肝, 结肠

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背景

ACSS2 (acyl-CoA synthetase short-chain family member 2), also known as ACAS2, ACS, ACSA or AceCS, is a 701 amino acid cytoplasmic protein that belongs to the ATP-dependent AMP-binding enzyme family. Existing as a monomer, ACSS2 functions to catalyze the ATP-dependent activation of acetate, a reaction that yields acetyl-CoA for use in energy generation and lipid synthesis. ACSS2 expression, which is highest in liver and kidney tissue, is regulated by the presence of unsaturated fatty acids and sterol regulatory element-binding proteins (SREBPs).

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图像

WB检测ACSS2蛋白(货号 YB112335). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.