产品信息

蛋白质全称	脂肪酸结合蛋白
别名	FABP6, Fatty acid binding protein 6, GT, I 15P, I BABP, I BALB, I BAP, ILBP, ILBP3, Ileal lipid binding protein, ILLBP, Intestinal 15 kDa protein
Uniprot ID	P51162
免疫原	小鼠FABP6重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	14 kDa / 14 kDa
纯化方式	亲和纯化
亚细胞定位	细胞质, 细胞膜

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 300-1: 500	小肠, 结肠
IHC/IF 免疫组织化学/免疫荧光	小鼠	1: 500-1: 1000	回肠

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背景

Fatty acid binding protein 6 (FABP6, also known as the ileal bile acid binding protein IBABP) is regarded as a bile acid binding protein found in the distal portion of the small intestine and may be important in maintaining bile acid homeostasis. FABP6 is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer. And also, it was showed a potential drug target for the treatment of diabetes. There are 2 isoforms of this protein,one of which is about 14 kDa we detected.

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图像

	WB检测FABP6蛋白(货号 YB113156). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.
	IHC检测FABP6蛋白(货号 YB113156). 样品: 小鼠回肠, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.