产品信息

蛋白质全称	酰基辅酶A硫酯酶1
别名	Acyl-CoA thioesterase 1, CTE-I, Inducible cytosolic acyl-coenzyme A thioester hydrolase, Long chain acyl-CoA hydrolase, Palmitoyl-coenzyme A thioesterase, Acot1, Cte1
Uniprot ID	O55137
免疫原	小鼠ACOT1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	46 kDa / 46 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 3000-1: 5000	心, 肝, 肾

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背景

Acyl-CoA thioesterases such as ACOT1, hydrolyze acyl-CoAs to the free fatty acid and CoA. ACOTs therefore play key roles in maintaining the intracellular ratio between CoA esters of various lipids and free fatty acids. Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.

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图像

WB检测ACOT1蛋白(货号 YB112168). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 3000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.