产品信息

蛋白质全称	肿瘤抑制候选蛋白3
别名	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3, ligosaccharyl transferase subunit TUSC3, Magnesium uptake/transporter TUSC3, Protein N33, Tusc3, N33
Uniprot ID	Q8BTV1
免疫原	小鼠TUSC3重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	40 kDa / 40 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 脊髓, 脑下垂体

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背景

TUSC3 (tumor suppressor candidate 3), originally named N33, is a potential tumor supressor gene. Decreased expression of TUSC3 has been found in various cancers, including prostate cancer, pancreas cancer and ovary cancer. TUSC3 also known as OST3A, is identified as a part of the oligosaccharyl-transferase (OST) complex and plays a crucial role in protein N-glycosylation. TUSC3 mutations have been found in families with non-syndromic autosomal recessive mental retardation.

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图像

WB检测TUSC3蛋白(货号 YB111832). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.