Anti-CD86 Rabbit pAb
- 50 μL
产品信息
蛋白质全称 | T淋巴细胞活化抗原CD86 |
别名 | Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 (C-terminal), CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72 |
免疫原 | 人CD86重组蛋白 |
Uniprot ID | P42081 |
抗体亚型 | IgG |
纯化方式 | 亲和纯化 |
预测分子量. / 观测分子量. | 37 kDa / 65-85 kDa |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人 | 1: 300-1: 600 | Raji, NAML6, Daudi |
背景
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4.May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced.Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.; [Isoform 2]: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.(Microbial infection) Acts as a receptor for adenovirus subgroup B.
图像
| WB检测CD86蛋白(货号 GB115630). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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