产品信息

蛋白质全称	精氨酸酶1
别名	Arg1, Arg-1, Arginase 1, Liver-Type Arginase, Arginase, Type I Arginase, Arginase-1
Uniprot ID	Q61176
免疫原	KLH偶联的小鼠Arginase 1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	35 kDa / 35-40 kDa
纯化方式	亲和纯化
亚细胞定位	细胞质, 细胞核

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 3000	肝, 邻近肝癌
IHC/IF 免疫组织化学/免疫荧光	小鼠, 大鼠	1: 500-1: 1500	肝脏, 受损皮肤

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背景

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia .

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图像

	WB检测Arginase 1蛋白(货号 GB115724). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测Arginase 1蛋白(货号 GB115724). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 人肝, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 小鼠受损皮肤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 小鼠肝, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 小鼠肺损伤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 大鼠受损皮肤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 大鼠缺血再灌注损伤心脏, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 大鼠肝, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IHC检测Arginase 1蛋白(货号 GB115724). 样品: 大鼠肺损伤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: S-vision免疫组化多聚二抗(山羊抗兔),即用型 (货号G1302), 室温孵育20分钟.
	IF analysis of Arginase 1 (GB115724) yellow. sample: Mouse damaged skin, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: Arginase 1 (GB115724) yellow 1: 2500, 4℃ overnight.HRP Goat Anti-Rabbit lgG (GB23303)+iF647-Tyramide(G1232). CD68 (GB113109) red 1: 1000, 4℃ overnight, Cy3 conjugated Goat Anti-Rabbit IgG (21303)+iF546-Tyramide(G1251).
	IF analysis of Arginase 1 (GB115724) yellow. sample: Mouse In situ tumor model of Lewis cell, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: Arginase 1 (GB115724) yellow 1: 2500, 4℃ overnight.HRP Goat Anti-Rabbit lgG (GB23303)+iF647-Tyramide(G1232). CD68 (GB113109) red 1: 1000, 4℃ overnight, Cy3 conjugated Goat Anti-Rabbit IgG (21303)+iF546-Tyramide(G1251).
	IF analysis of Arginase 1 (GB115724) yellow. sample: Mouse lung injury, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: Arginase 1 (GB115724) yellow 1: 2500, 4℃ overnight.HRP Goat Anti-Rabbit lgG (GB23303)+iF647-Tyramide(G1232). CD68 (GB113109) red 1: 1000, 4℃ overnight, Cy3 conjugated Goat Anti-Rabbit IgG (21303)+iF546-Tyramide(G1251).
	IF analysis of Arginase 1 (GB115724) yellow. sample: Rat lung injury, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: Arginase 1 (GB115724) yellow 1: 2500, 4℃ overnight.HRP Goat Anti-Rabbit lgG (GB23303)+iF647-Tyramide(G1232). CD68 (GB113109) red 1: 1000, 4℃ overnight, Cy3 conjugated Goat Anti-Rabbit IgG (21303)+iF546-Tyramide(G1251).

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.