产品信息

蛋白质全称	增强型绿色荧光蛋白
别名	Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP
Uniprot ID	P42212
免疫原	EGFP( enhanced green fluorescent protein)重组蛋白
抗体亚型	IgG1, κ
克隆号	2H5D1
预测分子量./观测分子量.	27 kDa / 29 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	Species independent	1: 500-1: 1000	细胞/组织中含有eGFP
ICC/IF 免疫细胞化学/免疫荧光	Species independent	1: 2000-1: 4000	CHO-S细胞

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背景

Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.

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图像

	WB检测EGFP蛋白(货号 GB12602). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.
	ICC检测EGFP蛋白(货号 GB12602)(红色). 样品: CHO-S细胞, 4%多聚甲醛 (货号G1101) 固定20分钟. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 一抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗小鼠IgG (H+L) (货号GB21301), 1: 300稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.