产品信息

蛋白质全称	T细胞表面糖蛋白CD4
别名	CD4, CD4mut, CD4 molecule
Uniprot ID	P06332
免疫原	KLH偶联的小鼠CD4合成肽
抗体亚型	IgG, κ
克隆号	SB1
预测分子量./观测分子量.	51 kDa / 55 kDa
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	胸腺, 淋巴结
IHC/IF 免疫组织化学/免疫荧光	小鼠	1: 200-1: 600	肺从急性肺损伤, 肺癌异位皮下肿瘤, 脾

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背景

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

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图像

	WB检测CD4蛋白(货号 GB15064). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	IHC检测CD4蛋白(货号 GB15064). 样品: 小鼠Lewis肺癌异位皮下瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液 (pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD4蛋白(货号 GB15064). 样品: 小鼠急性肺损伤肺组织, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液 (pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD4蛋白(货号 GB15064). 样品: 小鼠发炎皮肤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液 (pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD4蛋白(货号 GB15064). 样品: 小鼠脾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液 (pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 200稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD4蛋白(货号 GB15064). 样品: 小鼠脾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液 (pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 200稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD4蛋白(货号 GB15064). 样品: 小鼠Lewis肺癌异位皮下瘤, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液 (pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 200稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IF analysis of CD4(GB15064) red. sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD4(GB15064) red 1: 500, 4℃ overnight.Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. CD3 (GB12014) green 1: 500, 4℃ overnight,488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of CD4(GB15064) red. sample: Mouse subcutaneous nude-mouse model of 4T1 cell, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD4(GB15064) red 1: 500, 4℃ overnight.Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. CD3 (GB12014) green 1: 500, 4℃ overnight,488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of CD4(GB15064) red. sample: Mouse subcutaneous nude-mouse model of Lewis cell, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD4(GB15064) red 1: 500, 4℃ overnight.Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. CD3 (GB12014) green 1: 500, 4℃ overnight,488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.