产品信息

蛋白质全称	巨噬细胞甘露糖受体1
别名	CD206, CLEC13D, Macrophage mannose receptor 1, Mannose receptor, mannose receptor, C type 1, MMR, MRC1
Uniprot ID	P22897
免疫原	人CD206重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	内体膜, 细胞膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人	1: 300-1: 600	肺, 肝, 肺腺癌

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背景

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane glycoprotein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose).

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图像

	IHC检测Mannose Receptor/CD206蛋白(货号 GB115273). 样品: 人肺, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测Mannose Receptor/CD206蛋白(货号 GB115273). 样品: 人肺腺癌, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测Mannose Receptor/CD206蛋白(货号 GB115273). 样品: 人肝, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IF analysis of CD206(GB115273)green. sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD68(GB113150) red 1: 1000, 4℃ overnight, iF555-TSA(G1233). CD206(GB115273) green 1: 300, 4℃ overnight.488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.
	IF analysis of CD206(GB115273)green. sample: Human lung adenocarcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD68(GB113150) red 1: 1000, 4℃ overnight, iF555-TSA(G1233). CD206(GB115273) green 1: 300, 4℃ overnight.488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.