Recombinant Anti-Flag Tag antibody (Mouse mAb)
- 100 μL
产品信息
蛋白质全称 | Flag标签 |
别名 | DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG |
免疫原 | KLH偶联的DYKDDDDK合成肽 |
抗体亚型 | IgG1, κ |
克隆号 | SB17 |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | Species independent | 1: 500-1: 2000 | Flag-Tag融合蛋白 |
| ICC/IF 免疫细胞化学/免疫荧光 | Species independent | 1: 500-1: 1000 | 转染的HEK-293T细胞 |
| IP 免疫沉淀 | Species independent | 1: 50-1: 200 | 转染的HEK-293T细胞 |
背景
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
图像
| WB检测Flag Tag(货号 GB15938). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时. |
| 免疫共沉淀转染的HEK-293F细胞裂解液，使用Flag标签抗体（免疫沉淀抗体：GB15938，1:100；检测抗体：GB11938，1:3000） |
| ICC检测Flag Tag(货号 GB15938)(红色）. 样品: HEK-293T细胞, 4%多聚甲醛 (货号G1101) 固定20分钟. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗小鼠IgG (H+L) (货号GB21301), 1: 300稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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