产品信息

蛋白质全称	T细胞表面糖蛋白CD8α链
别名	T-lymphocyte differentiation antigen T8/Leu-2, CD8a, MAL, Leu2, Leu2 T lymphocyte antigen, Ly 35, LYT3, p32, T8 T cell antigen, CD8A, CD8, Leu2, p32, CD8a molecule, CD8 alpha
Uniprot ID	P01731
免疫原	KLH偶联的小鼠CD8 alpha合成肽
抗体亚型	IgG, κ
克隆号	SB67
预测分子量./观测分子量.	27 kDa / 33-37 kDa
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	胸腺, 脾
IHC/IF 免疫组织化学/免疫荧光	小鼠	1: 400-1: 800	4T1乳腺肿瘤模型, 结肠, Lewis皮下肿瘤模型

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背景

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs).

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图像

	WB检测CD8 alpha蛋白(货号 GB15068). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	IHC检测CD8 alpha蛋白(货号 GB15068). 样品: 小鼠4T1细胞皮下肿瘤模型, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD8 alpha蛋白(货号 GB15068). 样品: 小鼠结肠, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测CD8 alpha蛋白(货号 GB15068). 样品: 小鼠Lewis细胞皮下肿瘤模型, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IF analysis of CD8(GB15068) red. sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD8(GB15068) red 1: 500, 4℃ overnight.Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. CD3 (GB12014) green 1: 500, 4℃ overnight,488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of CD8(GB15068) red. sample: Mouse subcutaneous nude-mouse model of 4T1 cell, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD8(GB15068) red 1: 500, 4℃ overnight.Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. CD3 (GB12014) green 1: 500, 4℃ overnight,488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.
	IF analysis of CD8(GB15068) red. sample: Mouse subcutaneous nude-mouse model of Lewis cell, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD8(GB15068) red 1: 500, 4℃ overnight.Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. CD3 (GB12014) green 1: 500, 4℃ overnight,488 conjugated Goat Anti-Mouse IgG (H+L)（GB25301), 1: 400, RT, 1h.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.