产品信息

蛋白质全称	己糖激酶HKDC1
别名	Hexokinase domain-containing protein 1, Hkdc1, Putative hexokinase HKDC1
Uniprot ID	Q91W97
免疫原	KLH偶联的小鼠HKDC1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	102 kDa / 102 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	肝, 肾

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背景

Catalyzes the phosphorylation of hexose to hexose 6-phosphate, although at very low level compared to other hexokinases (By similarity). Has low glucose phosphorylating activity compared to other hexokinases . Involved in glucose homeostasis and hepatic lipid accumulation . Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role .

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图像

WB检测HKDC1蛋白(货号 YB111954). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.