产品信息

蛋白质全称	成纤维细胞生长因子6
别名	FGF-6, Heparin secretory-transforming protein 2, HST-2, HSTF-2, Heparin-binding growth factor 6, HBGF-6, Fgf6, Fgf-6, Hstf2
Uniprot ID	P21658
免疫原	小鼠FGF6重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	23 kDa / 23 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	脑, 心

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背景

FGF6 is a secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4. The FGF6 gene displays oncogenic transforming activity when transfected into mammalian cells.

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图像

WB检测FGF6蛋白(货号 GB113074). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.