产品信息

蛋白质全称	血小板衍生生长因子受体α
别名	PDGF-R-alpha, PDGFR alpha, CD140a, Pdgfra, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, Platelet-derived growth factor alpha receptor
Uniprot ID	P26618
免疫原	小鼠PDGFR alpha重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	123 kDa / 190 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	心, 胚胎

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背景

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

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图像

WB检测PDGFR alpha蛋白(货号 GB111342). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.