产品信息

蛋白质全称	盘状大同源蛋白2
别名	Channel-associated protein of synapse-110, Chapsyn-110, Postsynaptic density protein PSD-93, DLG2, channel-associated protein of synapses, Chapsyn-1, discs large 2, discs large homolog 2, discs, Post Synaptic Density 93, Postsynaptic density protein PSD-93
Uniprot ID	Q91XM9
免疫原	小鼠PSD93重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	98 kDa / 110 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HeLa细胞, SH-SY5Y细胞, Jurkat细胞

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背景

Chapsyn-110, is a part of the membrane-associated putative guanylate kinase (MAGUK) family. It binds directly to the NMDA receptor, and Shaker K+ channel subunits, and is 70-80% identical to PSD-95/SAP90 and SAP97. It associated tightly with the postsynaptic density I brain, and mediates the clustering of both NMDA recpetors and K+ channels in heterologous cells. The encoded protein forms a heterodimer with PSD-95 that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins.

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图像

WB检测PSD93蛋白(货号 GB111562). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.