产品信息

蛋白质全称	膜结合型前神经调节蛋白-1
别名	Pro-NRG1, Neuregulin-1, ARIA, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, HRGA, NDF, SMDF, HGL,HRG, Neu differentiation factor, Sensory and motor neuron-derived factor, NRG1, GGF
Uniprot ID	Q02297
免疫原	人NRG1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	70 kDa / 55 (mature); 72 (precursor) kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	肺

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背景

The NRG1 was originally identified as a glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure.

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图像

WB检测NRG1蛋白(货号 GB111258). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.