产品信息

蛋白质全称	膜初级胺氧化酶
别名	Copper amine oxidase, SSAO, HPAO, VP97, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, Vap1, Aoc3,
Uniprot ID	O70423
免疫原	KLH偶联的小鼠VAP1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	85 kDa / 100 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	小肠, 肾, 卵巢

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背景

VAP1(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, AOC3 and belongs to the copper topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions . VAP1 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated . VAP1 has molecular mass of 70-90kD, and can exsit as a homodimer and trimer.

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图像

	WB检测VAP1蛋白(货号 GB111007). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测VAP1蛋白(货号 GB111007). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.