产品信息

蛋白质全称	线粒体丝氨酸蛋白酶HTRA2
别名	High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA like serine protease, HtrA serine peptidase 2, Serine protease htra2 mitochondrial precursor, PARK 13, Protease serine 25
Uniprot ID	Q9JIY5
免疫原	小鼠HtrA2重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	49 kDa / 39 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	293T细胞, HeLa细胞, MCF7细胞

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背景

This (HtrA2)/Omi is a serine protease with homology to the E.coli HtrA protein (DegP) and is thought to be involved inapoptosis and stress induced degradation of misfolded proteins. While HtrA2 was orignally identified to bepresent in either the nucleus or endoplasmic reticulum , subsequent studies have shown that it localizes in mitochondria and is released during apoptosis. HtrA2 is produced as a 50 kDa zymogen that is cleaved to generate a 36 kDa mature protein that exposes an amino terminal motif (AVPS) resembling that of the IAP inhibitor Smac/Diablo.

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图像

WB检测HtrA2/Omi蛋白(货号 GB11982). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.