产品信息

蛋白质全称	微管解聚蛋白
别名	STMN1, C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, stathmin 1
Uniprot ID	P54227
免疫原	KLH偶联的小鼠Stathmin 1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	17 kDa / 18 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠	1: 300-1: 600	睾丸

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背景

Stathmin is a highly conserved 17 kDa protein that is crucial for the regulation of the cell cytoskeleton. The cytoskeleton is made up of long hollow cylinders named microtubules. These microtubules are made up of alpha and beta tubulin heterodimers. The changes in cytoskeleton are known as microtubule dynamics; the addition of the tubulin subunits lead to polymerisation and their loss, depolymerisation. Stathmin regulates these by promoting depolymerization of microtubules or preventing polymerization of tubulin heterodimers.

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图像

WB检测Stathmin 1蛋白(货号 GB115517). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.