产品信息

蛋白质全称	过氧化物还原酶2
别名	Thiol-specific antioxidant protein, TSA, Thioredoxin peroxidase 1, Prdx2, Tdpx1, TpxThioredoxin-dependent peroxide reductase 1, Thioredoxin-dependent peroxiredoxin 2, Thioredoxin peroxidase 1, Tpx, Peroxiredoxin 2
Uniprot ID	Q61171
免疫原	KLH偶联的小鼠  Peroxiredoxin 2合成肽
抗体亚型	IgG
预测分子量./观测分子量.	22 kDa / 22 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 2000	心, 肾, 脑

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背景

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.

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图像

WB检测Peroxiredoxin 2/PRP蛋白(货号 GB111254). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.