产品信息

蛋白质全称	神经轴突导向蛋白B1
别名	EFL-3, ELK-L, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EFNB1, EFL3, EPLG2, LERK2
Uniprot ID	P52795
免疫原	小鼠Ephrin B1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	38 kDa / 45 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	PC3细胞, HaCaT细胞, HepG2细胞

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背景

Ephrin-B1, also known as LERK-2, ELK-L, EFL-3, Cek5-L, and STRA-1 , is a member of the ephrin ligand family which binds members of the Eph receptor family. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse Ephrin-B1 share 94% amino acid identity.

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图像

WB检测Ephrin B1蛋白(货号 GB111417). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.