产品信息

蛋白质全称	Gem相关蛋白2
别名	Gemin2, GEMIN2, SIP1, SIP1-delta, Survival of motor neuron protein-interacting protein 1, gem nuclear organelle associated protein 2
Uniprot ID	Q9CQQ4
免疫原	KLH偶联的小鼠Gemin 2合成肽
抗体亚型	IgG
预测分子量./观测分子量.	30 kDa / 30 kDa
纯化方式	亲和纯化
亚细胞定位	细胞核

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	骨骼肌, 睾丸
IHC/IF 免疫组织化学/免疫荧光	小鼠, 大鼠	1: 500-1: 2000	睾丸

---

背景

This gene encodes one of the proteins found in the SMN complex, which consists of several gemini proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

---

图像

	WB检测Gemin 2蛋白(货号 GB11862). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	IHC检测Gemin 2蛋白(货号 GB11862). 样品: 大鼠睾丸, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.