产品信息

蛋白质全称	乙酰胆碱受体β亚基
别名	ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS, Nicotinic acetylcholine receptor beta subunit precursor
Uniprot ID	P09690
免疫原	KLH偶联的小鼠Nicotinic Acetylcholine Receptor beta/CHRNB1合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	小鼠, 大鼠	1: 1000-1: 2000	小脑

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背景

Acetylcholine receptors (AChRs) are integral membrane proteins that respond to the binding of acetylcholine (ACh), a neurotransmitter synthesized, stored and finally released by cholinergic neurons. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) subunits.

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图像

	IHC检测Nicotinic Acetylcholine Receptor beta/CHRNB1蛋白(货号 GB113337). 样品: 小鼠小脑, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测Nicotinic Acetylcholine Receptor beta/CHRNB1蛋白(货号 GB113337). 样品: 大鼠小脑, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.