产品信息

蛋白质全称	谷氨酸脱羧酶1
别名	Gad1, GAD1, CPSQ1, GAD, SCP, glutamate decarboxylase 1
Uniprot ID	P48318
免疫原	KLH偶联的小鼠GAD1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	67 kDa / 67 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 大脑皮层, 小脑

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背景

Glutamate decarboxylase 1 (brain, 67kDa) (GAD67), also known as GAD1, is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. This enzyme is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.

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图像

WB检测GAD1蛋白(货号 GB11717). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.