产品信息

蛋白质全称	NAD依赖性蛋白脱乙酰酶sirtuin-6
别名	SIR2 like protein 6, SIR2L6, SIRT6
Uniprot ID	P59941
免疫原	KLH偶联的小鼠SIRT6合成肽
抗体亚型	IgG
预测分子量./观测分子量.	37 kDa / 40 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 500	HeLa细胞, K562细胞

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背景

NAD-dependent protein deacetylase involved in various processes including telomere maintenance and gene expression, and consequently has roles in genomic stability, cell senescence and apoptosis Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate (By similarity). Has deacetylase activity towards histone H3K9Ac and H3K56Ac Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle (By similarity). May also be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance (By similarity). Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes

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图像

WB检测SIRT6蛋白(货号 GB115525). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.