产品信息

蛋白质全称	丝氨酸/苏氨酸蛋白激酶SIK1
别名	HRT-20, Myocardial SNF1-like kinase, Serine/threonine-protein kinase SNF1-like kinase 1, Salt-inducible kinase 1, SIK1
Uniprot ID	Q60670
免疫原	小鼠SIK1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	85 kDa / 90 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	胃, 肺, 卵巢

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背景

SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations. SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes. Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers.

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图像

WB检测SIK1蛋白(货号 GB111034). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.