产品信息

蛋白质全称	可能的全局转录激活因子SNF2L2
别名	ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, BAF190B, Protein brahma homolog, SNF2-alpha, Baf190b, Brm, Snf2a, Snf2l2, Smarca2
Uniprot ID	Q6DIC0
免疫原	KLH偶联的小鼠SMARCA2 / BRM合成肽
抗体亚型	IgG
预测分子量./观测分子量.	180 kDa / 250 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 350-1: 700	HepG2细胞, A431细胞, U-2OS细胞

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背景

A transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 / BRM belongs to the SNF2/RAD54 helicase family, is a homologue of the Saccharomyces cerevisiae SWI2/SNF2 and Drosophila brahma proteins. It contains a methyl lysine containing bromo domain and an HSA domain. The yeast protein SNF2, also known as SWI2, is involved in transcriptional activation of numerous genes. It contains a domain that is highly conserved among several known helicases and is required for transcriptional activity. SNF2/SWI2 is highly homologous to the Drosophila protein 'brahma' (brm).

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图像

WB检测SMARCA2/BRM蛋白(货号 GB112508). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 350稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.