产品信息

蛋白质全称	缺氧诱导因子3α
别名	HIF 3 alpha, HIF-3 Alpha, HIF 3A4, HIF3 alpha, HIF3A, HIF-3A, Hypoxia Inducible Factor 3 alpha, Hypoxia inducible factor 3 alpha subunit, Inhibitory PAS domain protein, IPAS, MOP7, PASD7
Uniprot ID	Q0VBL6
免疫原	KLH偶联的小鼠HIF3A合成肽
抗体亚型	IgG
预测分子量./观测分子量.	73 kDa / 73 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	MCF7细胞, A431细胞, HUVEC细胞

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背景

Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia).

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图像

WB检测HIF3A蛋白(货号 GB11844). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.