Anti-HIF-2-alpha Rabbit pAb
- 100 μL
产品信息
蛋白质全称 | 缺氧诱导因子-2α |
别名 | Epas1, EPAS1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, Endothelial PAS domain protein 1, Hypoxia-inducible factor-2alpha, HIF2α, endothelial PAS domain protein 1 |
Uniprot ID | |
免疫原 | 小鼠HIF2α重组蛋白 |
抗体亚型 | IgG |
预测分子量./观测分子量. | 97 kDa / 120 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人 | 1: 500-1: 1000 | 厌氧HeLa细胞, 厌氧A549细胞 |
背景
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
图像
| WB检测HIF-2-alpha蛋白(货号 GB111338). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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