GAPDH
- 100ul
Product Information
Description | GAPDH rabbit polyclonal |
Protein full name | Glyceraldehyde-3-phosphate dehydrogenase |
Synonyms | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
Isotype | IgG |
Predicted MW. | 37kDa |
Observed MW. | 37kDa |
Purity | Affinity purification |
Uniprot ID |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse, Rat | 1:1000-1:2000 | brain, spleen, kidney, liver |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Images
| Western blot analysis of GAPDH (GB11002) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF-7 cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Rat kidney tissue lysate Lane 9: Rat brain tissue lysate |
| Western blot analysis of GAPDH (GB11002) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat brain tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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