Anti-NF-κB p65 Rabbit pAb
- 100 μL
Product Information
Description | NFκB p65 rabbit polyclonal |
Protein full name | Transcription factor p65 |
Synonyms | RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit |
Immunogen | Recombinant protein corresponding to Mouse NFκB p65 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus,Cytoplasm |
Uniprot ID | Q04207,Q04206 |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, heart, kidney |
IHC | Human, Mouse, Rat | 1: 200-1: 400 | PC12, L6 |
IF | - | - | - |
Background
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.
Images
| Western blot analysis of NFkBp65 (GB11142) at dilution of 1:1000. Lane 1:HeLa cell lysate Lane 2:HepG2 cell lysate Lane 3:A549 cell lysate Lane 4:Mouse lung tissue lysate Lane 5:Mouse heart tissue lysate Lane 6:Mouse kidney tissue lysate Lane 7:Rat lung tissue lysate Lane 8:Rat heart tissue lysate Lane 9:Rat kidney tissue lysate |
| Immunofluorescence analysis of PC-12 cells,untreated(left) or treated with Rat Tumor Necrosis Factor-α(rTNF-α,20ng/ml,20min;right) using NFkBp65 (GB11142) at dilution of 1:200 |
| Immunofluorescence analysis of L6 cells,untreated(left) or treated with Rat Tumor Necrosis Factor-α(rTNF-α,20ng/ml,20min;right) using NFkBp65 (GB11142) at dilution of 1:200 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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